ARG56191
anti-Nucleolin antibody [NCL/902]
anti-Nucleolin antibody [NCL/902] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody [NCL/902] recognizes Nucleolin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | NCL/902 |
| Isotype | IgG1 |
| Target Name | Nucleolin |
| Antigen Species | Human |
| Immunogen | Recombinant human Nucleolin. |
| Conjugation | Un-conjugated |
| Alternate Names | Protein C23; C23; Nucleolin |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NCL |
| Gene Full Name | nucleolin |
| Background | Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008] |
| Function | Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. [UniProt] |
| Cellular Localization | Nucleoli |
| Calculated MW | 77 kDa |
| PTM | Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity). |
Images (9) Click the Picture to Zoom In
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ARG56191 anti-Nucleolin antibody [NCL/902] ICC/IF image
Immunofluorescence: HeLa cells stained with AF488-conjugated ARG56191 anti-Nucleolin antibody [NCL/902] (Green). Blue: DAPI.
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ARG56191 anti-Nucleolin antibody [NCL/902] FACS image
Flow Cytometry: Testing of Human 293T cells. Black: cells alone; Grey: Isotype control; Green: AF488-conjugated ARG56191 anti-Nucleolin antibody [NCL/902].
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ARG56191 anti-Nucleolin antibody [NCL/902] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil stained with ARG56191 anti-Nucleolin antibody [NCL/902].
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ARG56191 anti-Nucleolin antibody [NCL/902] WB image
Western blot: A431 cell lysate stained with ARG56191 anti-Nucleolin antibody [NCL/902].
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ARG56191 anti-Nucleolin antibody [NCL/902] FACS image
Flow Cytometry: Testing of Jurkat cells. Grey: Isotype control; Green: FITC-labeled; Purple: APC-Labeled; Turquoise: AF700-Labeled ARG56191 anti-Nucleolin antibody [NCL/902].
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ARG56191 anti-Nucleolin antibody [NCL/902] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human uterus stained with ARG56191 anti-Nucleolin antibody [NCL/902].
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ARG56191 anti-Nucleolin antibody [NCL/902] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human bladder carcinoma stained with ARG56191 anti-Nucleolin antibody [NCL/902].
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ARG56191 anti-Nucleolin antibody [NCL/902] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human ovarian carcinoma stained with ARG56191 anti-Nucleolin antibody [NCL/902].
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ARG56191 anti-Nucleolin antibody [NCL/902] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human testicular carcinoma stained with ARG56191 anti-Nucleolin antibody [NCL/902].
