ARG63263
anti-Npap60 / Nup50 antibody
anti-Npap60 / Nup50 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Gene Regulation antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Npap60 / Nup50 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_009103.2; NP_705931.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Npap60 / Nup50 |
| Antigen Species | Human |
| Immunogen | C-ELHKILLEKKDA |
| Conjugation | Un-conjugated |
| Alternate Names | Nuclear pore complex protein Nup50; 50 kDa nucleoporin; NPAP60L; Nucleoporin Nup50; NPAP60; Nuclear pore-associated protein 60 kDa-like |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9UKX7 Human Nuclear pore complex protein Nup50 |
|---|---|
| Background | The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Gene Regulation antibody; Signaling Transduction antibody |
| Calculated MW | 50 kDa |
Images (2) Click the Picture to Zoom In
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ARG63263 anti-Npap60 / Nup50 antibody WB image
Western Blot: Jurkat lysate (RIPA buffer, 30µg total protein per lane) stained with ARG63263 anti-Npap60 / Nup50 antibody at 0.5 µg/ml dilution.
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ARG63263 anti-Npap60 / Nup50 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Uterus. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63263 anti-Npap60 / Nup50 antibody at 3.8 µg/ml dilution followed by AP-staining.
