ARG62566
anti-Neutrophil Elastase antibody [NP57]
anti-Neutrophil Elastase antibody [NP57] for Immunohistochemistry,Western blot and Human
Microbiology and Infectious Disease antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [NP57] recognizes Neutrophil Elastase |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | NP57 |
| Isotype | IgG1, kappa |
| Target Name | Neutrophil Elastase |
| Antigen Species | Human |
| Immunogen | Human neutrophil granule proteins |
| Conjugation | Un-conjugated |
| Alternate Names | Neutrophil elastase; HNE; EC 3.4.21.37; Medullasin; NE; ELA2; GE; Bone marrow serine protease; SCN1; PMN-E; Human leukocyte elastase; HLE; Elastase-2; PMN elastase |
Application Instructions
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Properties
| Form | Liquid |
|---|---|
| Buffer | 1X PBS buffer with <0.1% sodium azide. |
| Preservative | <0.1% sodium azide. |
| Concentration | 2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ELANE |
| Gene Full Name | elastase, neutrophil expressed |
| Background | Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009] |
| Function | Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. [UniProt] |
| Research Area | Microbiology and Infectious Disease antibody; Signaling Transduction antibody |
| Calculated MW | 29 kDa |
