ARG11130
anti-Neurofilament NF-L (C-ter) antibody [6H112]
anti-Neurofilament NF-L (C-ter) antibody [6H112] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow,Pig
Overview
| Product Description | Mouse Monoclonal antibody [6H112] recognizes Neurofilament NF-L (C-ter) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Cow, Pig |
| Tested Application | ICC/IF, IHC-Fr, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 6H112 |
| Isotype | IgG1 |
| Target Name | Neurofilament NF-L (C-ter) |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide around the C-terminal region of Human Neurofilament NF-L. (C-GEEEDTKESEEEEKKEESAGEEQVAKKKD) |
| Conjugation | Un-conjugated |
| Alternate Names | Neurofilament triplet L protein; 68 kDa neurofilament protein; CMT1F; NF68; NFL; CMT2E; Neurofilament light polypeptide; NF-L; PPP1R110 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | ~ 62 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified |
| Buffer | PBS, 5 mM Sodium azide and 50% Glycerol. |
| Preservative | 5 mM Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NEFL |
| Gene Full Name | neurofilament, light polypeptide |
| Background | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008] |
| Function | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. [UniProt] |
| Calculated MW | 62 kDa |
| PTM | O-glycosylated. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. Ubiquitinated in the presence of TRIM2 and UBE2D1. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG11130 anti-Neurofilament NF-L (C-ter) antibody [6H112] IHC-Fr image
Immunohistochemistry: Frozen section of Rat frontal cortex tissue stained with ARG11130 anti-Neurofilament NF-L (C-ter) antibody [6H112] (green) at 1:2000 dilution, and co-stained with ARG10712 anti-FOX3 / NeuN antibody (red) at 1:2000 dilution. (Sample preparation: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with the above antibodies.).
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ARG11130 anti-Neurofilament NF-L (C-ter) antibody [6H112] WB image
Western blot: Rat spinal cord, Mouse spinal cord, Pig spinal cord and Cow spinal cord lysates stained with ARG11130 anti-Neurofilament NF-L (C-ter) antibody [6H112] at 1:5000 dilution.
