ARG11132
anti-Neurofilament NF-H phospho (KSP site) antibody [9B12]
anti-Neurofilament NF-H phospho (KSP site) antibody [9B12] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow
Overview
| Product Description | Mouse Monoclonal antibody [9B12] recognizes Neurofilament NF-H phospho (KSP site) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Cow |
| Tested Application | ICC/IF, IHC-Fr, WB |
| Specificity | This antibody recognizes the phosphorylated NF-H KSP sequences. In some species there is some cross-reactivity with the phosphorylated KSP sequences found in the related neurofilament subunit NF-M. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 9B12 |
| Isotype | IgG2b |
| Target Name | Neurofilament NF-H |
| Antigen Species | Bovine |
| Immunogen | Native Neurofilament NF-H purified from bovine spinal cord, binding to phosphorylated KSP sequences. |
| Conjugation | Un-conjugated |
| Alternate Names | Neurofilament heavy polypeptide; 200 kDa neurofilament protein; NF-H; Neurofilament triplet H protein; NFH |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified |
| Buffer | PBS, 5 mM Sodium azide and 50% Glycerol. |
| Preservative | 5 mM Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NEFH |
| Gene Full Name | neurofilament, heavy polypeptide |
| Background | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008] |
| Function | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Calculated MW | 112 kDa |
| PTM | There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG11132 anti-Neurofilament NF-H phospho (KSP site) antibody [9B12] ICC/IF image
Immunofluorescence: E20 rat cortex cells stained with ARG11132 anti-Neurofilament NF-H phospho (KSP site) antibody [9B12] (red) at 1:5000 dilution, and co-stained with ARG10732 anti-Neurofilament NF-L antibody antibody (green) at 1:5000 dilution. DAPI (blue) for nuclear staining.
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ARG11132 anti-Neurofilament NF-H phospho (KSP site) antibody [9B12] IHC-Fr image
Immunohistochemistry: Frozen section of Rat brain coronal tissue of the third ventricle stained with ARG11132 anti-Neurofilament NF-H phospho (KSP site) antibody [9B12] (green) at 1:5000 dilution. Hoechst (blue) for nuclear staining. (Sample preparation: Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibody.).
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ARG11132 anti-Neurofilament NF-H phospho (KSP site) antibody [9B12] WB image
Western blot: Rat spinal cord, Mouse spinal cord and Cow spinal cord lysates stained with ARG11132 anti-Neurofilament NF-H phospho (KSP site) antibody [9B12] at 1:10000 dilution.
Smaller proteolytic fragments of NF-H are also detected in some preparations.
