ARG40709
anti-Nestin antibody
anti-Nestin antibody for IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; NSC antibody; Neural Stem Cell Marker antibody; NSC and Neuron Marker antibody; Neuron Development Study antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Nestin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-Fr, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Nestin |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 252-290 of Human Nestin. (ERLRATEKFQLAVEALEQEKQGLQSQIAQVLEGRQQLAH) |
| Conjugation | Un-conjugated |
| Alternate Names | NES; Nestin FLJ21841; Nbla00170 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NES |
| Gene Full Name | nestin |
| Background | This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. |
| Function | Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. |
| Cellular Localization | Intermediate filament |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; NSC antibody; Neural Stem Cell Marker antibody; NSC and Neuron Marker antibody; Neuron Development Study antibody |
| Calculated MW | 177 kDa |
| PTM | Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation |
Images (6) Click the Picture to Zoom In
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ARG40709 anti-Nestin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat kidney tissue stained with ARG40709 anti-Nestin antibody at 1 µg/ml dilution.
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ARG40709 anti-Nestin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human melanoma tissue stained with ARG40709 anti-Nestin antibody at 1 µg/ml dilution.
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ARG40709 anti-Nestin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat kidney tissue stained with ARG40709 anti-Nestin antibody at 1 µg/ml dilution.
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ARG40709 anti-Nestin antibody IHC-Fr image
Immunohistochemistry: Frozen section of Mouse kidney tissue. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40709 anti-Nestin antibody at 1 μg/ml dilution, overnight at 4°C.
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ARG40709 anti-Nestin antibody IHC-Fr image
Immunohistochemistry: Frozen section of Rat kidney tissue. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40709 anti-Nestin antibody at 1 μg/ml dilution, overnight at 4°C.
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ARG40709 anti-Nestin antibody WB image
Western blot: HeLa and HepG2 whole cell lysates stained with ARG40709 anti-Nestin antibody at 0.5 µg/ml dilution.
