ARG10731
anti-Nestin antibody
anti-Nestin antibody for ICC/IF,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; NSC antibody; Neural Stem Cell Marker antibody; NSC and Neuron Marker antibody; Neuron Development Study antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Nestin |
|---|---|
| Tested Reactivity | Hu |
| Species Does Not React With | Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Nestin |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around aa. 315-630 of Human Nestin. |
| Conjugation | Un-conjugated |
| Alternate Names | NES; Nestin FLJ21841; Nbla00170 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Unpurified. |
| Buffer | Serum. |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NES |
| Gene Full Name | nestin |
| Background | This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. |
| Function | Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; NSC antibody; Neural Stem Cell Marker antibody; NSC and Neuron Marker antibody; Neuron Development Study antibody |
| Calculated MW | 177 kDa |
| PTM | Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized (By similarity). |
Images (4) Click the Picture to Zoom In
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ARG10731 anti-Nestin antibody ICC/IF image
Immunocytochemistry: SH-SY5Y neuroblastoma cells were stained with ARG10731 anti-Nestin antibody at 1:5000 (red) and co-stained with an antibody against vimentin at 1:10000 (green). Vimentin is main partner of nestin forming heterdimers and further polymerizing to form intermediate filaments. Co-localization of vimentin and nestin in cells was shown in yellow. Blue is DAPI staining of DNA.
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ARG10731 anti-Nestin antibody ICC/IF image
Immunofluorescence: SH-SY5Y cells stained with ARG10731 anti-Nestin antibody (green) at 1:3000 dilution and costained with Mouse mAb to actin (red) at 1:500 dilution. Hoechst (blue) for nuclear staining.
The Nestin antibody produces strong staining in the cytoplasm in clearly filamentous fashion, while actin antibody stains the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites.
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ARG10731 anti-Nestin antibody WB image
Western blot: SH-SY5Y homogenate stained with ARG10731 anti-Nestin antibody at 1:2000 dilution.
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ARG10731 anti-Nestin antibody WB image
Western blot: Rat cortical neuron-glial cell and SH-SY5Y cell lysates stained with ARG10731 anti-Nestin antibody (green) at 1:3000 dilution.
The blot detected only in Human cells.
