ARG10579
anti-Nephrin antibody [Y17-R]
anti-Nephrin antibody [Y17-R] for ICC/IF,Western blot and Human,Mouse,Rat
Publication1
Overview
| Product Description | Rabbit Monoclonal antibody [Y17-R] recognizes Nephrin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | Y17-R |
| Isotype | IgG |
| Target Name | Nephrin |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the center of the fifth Ig-like domain of Human nephrin (between residues Cys465-Cys528) |
| Conjugation | Un-conjugated |
| Alternate Names | CNF; NPHN; Renal glomerulus-specific cell adhesion receptor; nephrin; Nephrin |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | 20 mM Tris-HCl (pH 8.0), 0.05% Sodium azide and 10 mg/ml BSA |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 10 mg/ml BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NPHS1 |
| Gene Full Name | nephrosis 1, congenital, Finnish type (nephrin) |
| Background | This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009] |
| Function | Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity). [UniProt] |
| Calculated MW | 135 kDa |
| PTM | Phosphorylated at Tyr-1193 by FYN, leading to the recruitment and activation of phospholipase C-gamma-1/PLCG1. |
Images (2) Click the Picture to Zoom In
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ARG10579 anti-Nephrin antibody [Y17-R] ICC/IF image
Immunocytochemistry: HEK293 cells stained with ARG10579 anti-Nephrin antibody [Y17-R] at 1:700 dilution.
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ARG10579 anti-Nephrin antibody [Y17-R] WB image
Western blot: 1) 50 ng, 2) 100 ng, and 3) 200 ng of recombinant Mouse nephrin (extracellular domain, Gln37 - Thr1049) stained with ARG10579 anti-Nephrin antibody [Y17-R].
Specific References
A glomerulus chip with spherically twisted cell-laden hollow fibers as glomerular capillary tufts
ICC/IF / Human
