ARG42345
anti-Nectin 2 antibody [R2.525] (PE)
anti-Nectin 2 antibody [R2.525] (PE) for Flow cytometry and Human
Overview
| Product Description | PE-conjugated Mouse Monoclonal antibody [R2.525] recognizes Nectin 2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS |
| Specificity | The mouse monoclonal antibody R2.525 recognizes an extracellular epitope on CD112, a type I transmembrane glycoprotein expressed by myelomonocytic and megakaryocytic cells, and by CD34+ hematopoietic progenitors. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | R2.525 |
| Isotype | IgG1, kappa |
| Target Name | Nectin 2 |
| Antigen Species | Human |
| Immunogen | NIH/3T3 cells transfected with Human Nectin 2. |
| Conjugation | PE |
| Alternate Names | PVRR2; Nectin-2; HveB; PRR2; HVEB; CD antigen CD112; CD112; Poliovirus receptor-related protein 2; Herpes virus entry mediator B; Herpesvirus entry mediator B |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified |
| Buffer | PBS and 15 mM Sodium azide. |
| Preservative | 15 mM Sodium azide |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PVRL2 |
| Gene Full Name | poliovirus receptor-related 2 (herpesvirus entry mediator B) |
| Background | This gene encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| Function | Modulator of T-cell signaling. Can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation. These interactions are competitive (PubMed:26755705). Probable cell adhesion protein (PubMed:9657005). (Microbial infection) Acts as a receptor for herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV). [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 58 kDa |
Images (1) Click the Picture to Zoom In
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ARG42345 anti-Nectin 2 antibody [R2.525] (PE) FACS image
Flow Cytometry: Separation of Caco-2 cells stained with ARG42345 anti-Nectin 2 antibody [R2.525] (PE) (10 µl reagent / 10^6 cells in 100 µl of cell suspension) (red-filled) from Caco-2 cells stained with ARG65337 Mouse IgG1 Kappa Isotype Control antibody [MOPC-21] (PE) at 5 µg/ml dilution (black-dashed).
