ARG41942

anti-NUP35 antibody

anti-NUP35 antibody for Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes NUP35
Tested Reactivity Hu, Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NUP35
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-326 of Human NUP35 (NP_612142.2).
Conjugation Un-conjugated
Alternate Names NUP53; Nuclear pore complex protein Nup53; MP44; Mitotic phosphoprotein 44; Nucleoporin NUP53; 35 kDa nucleoporin; MP-44; Nucleoporin Nup35; NP44

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Jurkat
Observed Size ~ 37 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 129401 Human NUP35

GeneID: 295692 Rat NUP35

GeneID: 69482 Mouse NUP35

Gene Symbol NUP35
Gene Full Name nucleoporin 35kDa
Background This gene encodes a member of the nucleoporin family. The encoded protein contains two membrane binding regions, is localized to the nuclear rim, and is part of the nuclear pore complex. All molecules entering or leaving the nucleus either diffuse through or are actively transported by the nuclear pore complex. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 7 and 10. [provided by RefSeq, Dec 2013]
Function Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs). Can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC. [UniProt]
Cellular Localization Nucleus, nuclear pore complex. Nucleus membrane; Peripheral membrane protein. Note=Tightly associated with the nuclear membrane and lamina. [UniProt]
Calculated MW 35 kDa

Images (1) Click the Picture to Zoom In

  • ARG41942 anti-NUP35 antibody WB image

    Western blot: 25 µg of Jurkat cell lysate stained with ARG41942 anti-NUP35 antibody at 1:3000 dilution.