ARG63278
anti-NUMB antibody
anti-NUMB antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes NUMB |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Cow, Dog |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet been tested experimentally. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NUMB |
| Antigen Species | Human |
| Immunogen | C-PFSSDLQKTFEIEL |
| Conjugation | Un-conjugated |
| Alternate Names | c14_5527; C14orf41; Protein S171; S171; h-Numb; Protein numb homolog |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Mouse brain | ||||||||
| Observed Size | ~ 76 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Background | The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Research Area | Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 71 kDa |
| PTM | Phosphorylated on Ser-276 and Ser-295 by CaMK1. Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity). Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation. |
Images (4) Click the Picture to Zoom In
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ARG63278 anti-NUMB antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed A431 cells permeabilized with 0.15% Triton. Cells were stained with ARG63278 anti-NUMB antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63278 anti-NUMB antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostate tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63278 anti-NUMB antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63278 anti-NUMB antibody WB image
Western blot: 35 µg of Mouse brain lysate (in RIPA buffer) stained with ARG63278 anti-NUMB antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
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ARG63278 anti-NUMB antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed NIH/3T3 cells permeabilized with 0.15% Triton. Cells were stained with ARG63278 anti-NUMB antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
