ARG63079
anti-NTAL / LAB antibody [NAP-07]
anti-NTAL / LAB antibody [NAP-07] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human,Mouse
Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [NAP-07] recognizes NTAL / LAB |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, ICC/IF, IP, WB |
| Specificity | The clone NAP-07 reacts with Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B lymphocytes, NK cells and myeloid cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | NAP-07 |
| Isotype | IgG1 |
| Target Name | NTAL / LAB |
| Antigen Species | Human |
| Immunogen | Recombinant cytoplasmic domain (aa 91-243) of human NTAL. |
| Conjugation | Un-conjugated |
| Alternate Names | Membrane-associated adapter molecule; Non-T-cell activation linker; WSCR5; WBSCR15; Linker for activation of B-cells; HSPC046; LAB; WBSCR5; NTAL; Williams-Beuren syndrome chromosomal region 15 protein; Linker for activation of T-cells family member 2; Williams-Beuren syndrome chromosomal region 5 protein |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Antibody incubation: 60 min. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing condition. 12% separating SDS-PAGE gel. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | FACS: Positive control: Raji and Ramos WB: Positive control: Ramos. Negative control: HeLa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9GZY6 Human Linker for activation of T-cells family member 2 Swiss-port # Q9JHL0 Mouse Linker for activation of T-cells family member 2 |
|---|---|
| Gene Symbol | LAT2 |
| Gene Full Name | linker for activation of T cells family, member 2 |
| Background | NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function. |
| Function | Involved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. May also be involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-mediated signaling in myeloid cells. Couples activation of these receptors and their associated kinases with distal intracellular events through the recruitment of GRB2. [UniProt] |
| Research Area | Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 27 kDa |
| PTM | Phosphorylated on tyrosines following cross-linking of BCR in B-cells, FCGR1 in myeloid cells, or FCER1 in mast cells; which induces the recruitment of GRB2. May be polyubiquitinated. |
Images (1) Click the Picture to Zoom In
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ARG63079 anti-NTAL / LAB antibody [NAP-07] ICC/IF image
Immunofluorescence: THP-1 cells stained with ARG63079 anti-NTAL / LAB antibody [NAP-07] (green).
Nuclei was stained with propidium iodide (red).
Clone References
Vav and Rac activation in B cell antigen receptor endocytosis involves Vav recruitment to the adapter protein LAB.
WB / Mouse
Regulation of Ca2+ signaling in mast cells by tyrosine-phosphorylated and unphosphorylated non-T cell activation linker.
Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells.
Comparative gene expression profiling of in vitro differentiated megakaryocytes and erythroblasts identifies novel activatory and inhibitory platelet membrane proteins.
Activation-induced endocytosis of the raft-associated transmembrane adaptor protein LAB/NTAL in B lymphocytes: evidence for a role in internalization of the B cell receptor.
