ARG44129

anti-NSUN3 antibody

anti-NSUN3 antibody for Flow cytometry,Western blot and Human,Monkey

Overview

Product Description Rabbit Polyclonal recognizes NSUN3
Tested Reactivity Hu, Mk
Tested Application FACS, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NSUN3
Antigen Species Human
Immunogen Human NSUN3 recombinant protein (Position: K12-E310).
Conjugation Un-conjugated
Alternate Names NSUN3; NOP2/Sun RNA Methyltransferase 3; TRNA (Cytosine(34)-C(5))-Methyltransferase, Mitochondrial; NOP2/Sun RNA Methyltransferase Family Member 3; NOL1/NOP2/Sun Domain Family Member 3; FLJ22609; NOL1/NOP2/Sun Domain Family, Member 3; Putative Methyltransferase NSUN3; EC 2.1.1.-; EC 2.1.1; COXPD48; MSTP077; MST077

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
WB0.25-0.5 µg/ml
Application Note The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 63899 Human NSUN3

Swiss-port # Q9H649 Human Putative methyltransferase NSUN3

Gene Symbol NSUN3
Gene Full Name NOP2/Sun RNA Methyltransferase 3
Background Enables tRNA (cytosine-5-)-methyltransferase activity. Involved in regulation of mitochondrial translation and tRNA wobble base cytosine methylation. Located in mitochondrial matrix. Implicated in combined oxidative phosphorylation deficiency 48.
Function Mitochondrial tRNA methyltransferase that mediates methylation of cytosine to 5-methylcytosine (m5C) at position 34 of mt-tRNA(Met).
Cellular Localization Mitochondrion
Calculated MW 38 kDa

Images (2) Click the Picture to Zoom In

  • ARG44129 anti-NSUN3 antibody WB image

    Western blot: COS-7 stained with ARG44129 anti-NSUN3 antibody at 0.5 μg/ml dilution.

  • ARG44129 anti-NSUN3 antibody FACS image

    Flow Cytometry: U251 stained with ARG44129 anti-NSUN3 antibody at 1 µg/10^6 cells dilution.