ARG44128
anti-NSUN2 antibody
anti-NSUN2 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal recognizes NSUN2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NSUN2 |
| Antigen Species | Human |
| Immunogen | Human NSUN2 recombinant protein (Position: H146-K654). |
| Conjugation | Un-conjugated |
| Alternate Names | NSUN2; NOP2/Sun RNA Methyltransferase 2; TRM4; SAKI; RNA Cytosine C(5)-Methyltransferase NSUN2; Misu; Myc-Induced SUN-Domain-Containing Protein; Myc-Induced SUN Domain-Containing Protein; NOL1/NOP2/Sun Domain Family, Member 2; MRNA Cytosine C(5)-Methyltransferase; TRNA Cytosine C(5)-Methyltransferase; Substrate Of AIM1/Aurora Kinase B; NOP2/Sun Domain Family, Member 2; TRNA Methyltransferase 4 Homolog; FLJ20303; MRT5; Mental Retardation, Non-Syndromic, Autosomal Recessive, 5; TRNA Methyltransferase 4 Homolog (S. Cerevisiae); NOP2/Sun RNA Methyltransferase Family Member 2; TRNA (Cytosine(34)-C(5))-Methyltransferase; TRNA (Cytosine-5-)-Methyltransferase NSUN2; NOL1/NOP2/Sun Domain Family Member 2; 5-Methycytoisine Methyltransferase; EC 2.1.1.203; EC 2.1.1.29; EC 2.1.1.-; EC 2.1.1; HTrm4; MISU |
Application Instructions
| Application Suggestion |
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| Application Note | The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q08J23 Human tRNA (cytosine(34)-C(5))-methyltransferase Swiss-port # Q1HFZ0 Mouse tRNA (cytosine(34)-C(5))-methyltransferase |
|---|---|
| Gene Symbol | NSUN2 |
| Gene Full Name | NOP2/Sun RNA Methyltransferase 2 |
| Background | This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. |
| Function | RNA cytosine C5-methyltransferase that methylates cytosine to 5-methylcytosine (m5C) in various RNAs, such as tRNAs, mRNAs and some long non-coding RNAs (lncRNAs). |
| Cellular Localization | Cytoplasm, Cytoskeleton, Mitochondrion, Nucleus, Secreted |
| Calculated MW | 86 kDa |
| PTM | Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation |
Images (7) Click the Picture to Zoom In
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ARG44128 anti-NSUN2 antibody IHC-P image
Immunohistochemistry: Human acinic cell carcinoma stained with ARG44128 anti-NSUN2 antibody at 2 μg/ml dilution.
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ARG44128 anti-NSUN2 antibody WB image
Western blot: Hela and 293T stained with ARG44128 anti-NSUN2 antibody at 0.5 μg/ml dilution.
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ARG44128 anti-NSUN2 antibody FACS image
Flow Cytometry: 293T stained with ARG44128 anti-NSUN2 antibody at 1 µg/10^6 cells dilution.
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ARG44128 anti-NSUN2 antibody WB image
Western blot: Rat brain stained with ARG44128 anti-NSUN2 antibody at 0.5 μg/ml dilution.
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ARG44128 anti-NSUN2 antibody WB image
Western blot: Mouse brain stained with ARG44128 anti-NSUN2 antibody at 0.5 μg/ml dilution.
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ARG44128 anti-NSUN2 antibody IHC-P image
Immunohistochemistry: Human colorectal adenocarcinoma stained with ARG44128 anti-NSUN2 antibody at 2 μg/ml dilution.
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ARG44128 anti-NSUN2 antibody IHC-P image
Immunohistochemistry: Human ovarian cancer stained with ARG44128 anti-NSUN2 antibody at 2 μg/ml dilution.
