ARG59944
anti-NSFL1C antibody
anti-NSFL1C antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NSFL1C |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NSFL1C |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-185 of Human NSFL1C (NP_057227.2). |
| Conjugation | Un-conjugated |
| Alternate Names | p97 cofactor p47; dJ776F14.1; UBXD10; UBX domain-containing protein 2C; NSFL1 cofactor p47; UBX1; UBXN2C; P47 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 41 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NSFL1C |
| Gene Full Name | NSFL1 (p97) cofactor (p47) |
| Background | N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8. [provided by RefSeq, May 2011] |
| Function | Reduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER) (By similarity). Inhibits the activity of CTSL (in vitro). [UniProt] |
| Cellular Localization | Nucleus. Golgi apparatus, Golgi stack. Chromosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Predominantly nuclear in interphase cells. Bound to the axial elements of sex chromosomes in pachytene spermatocytes. A small proportion of the protein is cytoplasmic, associated with Golgi stacks. Localizes to centrosome during mitotic prophase and metaphase. [UniProt] |
| Calculated MW | 41 kDa |
| PTM | Phosphorylated during mitosis. Phosphorylation inhibits interaction with Golgi membranes and is required for the fragmentation of the Golgi stacks during mitosis (By similarity). [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG59944 anti-NSFL1C antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG59944 anti-NSFL1C antibody.
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ARG59944 anti-NSFL1C antibody IP image
Immunoprecipitation: 200 µg extracts of A549 cells immunoprecipitated and stained with ARG59944 anti-NSFL1C antibody at 1:1000 dilution.
