ARG43621
anti-NSE / Neuron Specific Enolase antibody
anti-NSE / Neuron Specific Enolase antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NSE / Neuron Specific Enolase |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NSE / Neuron Specific Enolase |
| Antigen Species | Human |
| Immunogen | Synthetic peptide within a.a. 50-150 of Human NSE / Neuron Specific Enolase. |
| Conjugation | Un-conjugated |
| Protein Full Name | Gamma-enolase |
| Alternate Names | Neural enolase; NSE; Enolase 2; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; HEL-S-279; Neuron-specific enolase; EC 4.2.1.11 |
Application Instructions
| Application Suggestion |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||||||
| Observed Size | ~ 48 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ENO2 |
| Gene Full Name | enolase 2 (gamma, neuronal) |
| Background | This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008] |
| Function | Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity). [UniProt] |
| Cellular Localization | Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. [UniProt] |
| Calculated MW | 47 kDa |
| PTM | Acetylation; Hydroxylation; Isopeptide bond; Phosphoprotein; Ubl conjugation |
Images (7) Click the Picture to Zoom In
-
ARG43621 anti-NSE / Neuron Specific Enolase antibody WB image
Western blot: A431 and HepG2 stained with ARG43621 anti-NSE / Neuron Specific Enolase antibody at 0.5 μg/mL dilution.
-
ARG43621 anti-NSE / Neuron Specific Enolase antibody IHC-P image
Immunohistochemistry: Human pancreatic cancer stained with ARG43621 anti-NSE / Neuron Specific Enolase antibody at 1 μg/ml dilution.
-
ARG43621 anti-NSE / Neuron Specific Enolase antibody ICC/IF image
Immunofluorescence: A431 stained with ARG43621 anti-NSE / Neuron Specific Enolase antibody at 5 μg/ml dilution.
-
ARG43621 anti-NSE / Neuron Specific Enolase antibody FACS image
Flow Cytometry: A431 stained with ARG43621 anti-NSE / Neuron Specific Enolase antibody at 1 μg / 10^6 cells dilution.
-
ARG43621 anti-NSE / Neuron Specific Enolase antibody WB image
Western blot: Rat brain and Mouse brain stained with ARG43621 anti-NSE / Neuron Specific Enolase antibody at 0.5 μg/mL dilution.
-
ARG43621 anti-NSE / Neuron Specific Enolase antibody IHC-P image
Immunohistochemistry: Rat brain stained with ARG43621 anti-NSE / Neuron Specific Enolase antibody at 1 μg/ml dilution.
-
ARG43621 anti-NSE / Neuron Specific Enolase antibody IHC-P image
Immunohistochemistry: Mouse brain stained with ARG43621 anti-NSE / Neuron Specific Enolase antibody at 1 μg/ml dilution.
