ARG11115
anti-NSE / Neuron Specific Enolase antibody
anti-NSE / Neuron Specific Enolase antibody for IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow
Overview
| Product Description | Chicken Polyclonal antibody recognizes NSE / Neuron Specific Enolase |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Cow |
| Tested Application | IHC-Fr, WB |
| Host | Chicken |
| Clonality | Polyclonal |
| Isotype | IgY |
| Target Name | NSE / Neuron Specific Enolase |
| Antigen Species | Human |
| Immunogen | Recombinant full-length Human NSE / Neuron Specific Enolase. |
| Conjugation | Un-conjugated |
| Alternate Names | Neural enolase; NSE; Enolase 2; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; HEL-S-279; Neuron-specific enolase; EC 4.2.1.11 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | ~ 45 kDa |
Properties
| Form | Liquid |
|---|---|
| Buffer | PBS and 5 mM Sodium azide. |
| Preservative | 5 mM Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ENO2 |
| Gene Full Name | enolase 2 (gamma, neuronal) |
| Background | This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008] |
| Function | Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity). [UniProt] |
| Cellular Localization | Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. [UniProt] |
| Calculated MW | 47 kDa |
Images (2) Click the Picture to Zoom In
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ARG11115 anti-NSE / Neuron Specific Enolase antibody IHC-Fr image
Immunohistochemistry: Frozen section of adult mouse cerebellar dentate nucleus stained with ARG11115 anti-NSE / Neuron Specific Enolase antibody (red) at 1:3000 dilution. The section was co-stained with anti-GFAP antibody (green) at 1:5000 dilution. Hoechst (blue) for nuclear staining. (Sample preparation: Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies.).
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ARG11115 anti-NSE / Neuron Specific Enolase antibody WB image
Western blot: Rat brain, Mouse brain, Cow brain and SH-SY5Y cell lysates stained with ARG11115 anti-NSE / Neuron Specific Enolase antibody at 1:5000 dilution.
