ARG62512
anti-NRG1 / Heregulin beta 1 antibody [7D5]
anti-NRG1 / Heregulin beta 1 antibody [7D5] for IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Neuroscience antibody
Overview
| Product Description | Mouse Monoclonal antibody [7D5] recognizes NRG1 / Heregulin beta 1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-Fr, IHC-P, IP, WB |
| Specificity | This antibody reacts with a 44 kD protein known as NRG1 (Neuregulin 1)/ Heregulin/Neu Differentiation Factor/NDF. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 7D5 |
| Isotype | IgG2a |
| Target Name | NRG1 / Heregulin beta 1 |
| Antigen Species | Rat |
| Immunogen | Recombinant extracellular domain of rat NDF protein |
| Conjugation | Un-conjugated |
| Alternate Names | Sensory and motor neuron-derived factor; Heregulin; GGF2; Glial growth factor; Acetylcholine receptor-inducing activity; SMDF; ARIA; NRG1-IT2; Neu differentiation factor; HRGA; NDF; Breast cancer cell differentiation factor p45; HGL; GGF; MSTP131; Pro-NRG1; HRG; MST131; HRG1; Pro-neuregulin-1, membrane-bound isoform |
Application Instructions
| Application Note | WB: 1-5 μg/ml IP: 2 μl/mg of lysate IHC: 1/10-1/500 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
Properties
| Form | Liquid |
|---|---|
| Buffer | PBS (pH 7.4) and 0.08% Sodium azide |
| Preservative | 0.08% Sodium azide |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P43322 Rat Pro-neuregulin-1, membrane-bound isoform Swiss-port # Q02297 Human Pro-neuregulin-1, membrane-bound isoform |
|---|---|
| Gene Symbol | Nrg1 |
| Gene Full Name | neuregulin 1 |
| Background | The protein encoded by this gene is a membrane glycoprotein that that mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. An extraordinary variety of different isoforms are produced from this gene through alternative promoter usage and splicing. These isoforms are expressed in a tissue-specific manner and differ significantly in their structure, and are classified as types I, II, III, IV, V and VI. Dysregulation of this gene has been linked to diseases such as cancer, schizophrenia, and bipolar disorder (BPD). [provided by RefSeq, Jun 2014] |
| Function | Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vessicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart (By similarity). [UniProt] |
| Cellular Localization | Secreted; Cell membrane |
| Research Area | Neuroscience antibody |
| Calculated MW | 70 kDa |
| PTM | Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form. N- and O-glycosylated. Extensive glycosylation precedes the proteolytic cleavage (By similarity). |
Clone References
Neuregulin-1 (Nrg1) is mainly expressed in rat pituitary gonadotroph cells and possibly regulates prolactin (PRL) secretion in a juxtacrine manner.
WB / Rat
Endothelium-derived neuregulin protects the heart against ischemic injury.
WB / Human
Phenotypic characterization of transgenic mice overexpressing neuregulin-1.
WB / Mouse
Ventricular ErbB2/ErbB4 activation and downstream signaling in pacing-induced heart failure.
WB / Dog
Peripheral neuropathy in the twitcher mouse: accumulation of extracellular matrix in the endoneurium and aberrant expression of ion channels.
IHC / Mouse
NRG1 gene rearrangements in clinical breast cancer: identification of an adjacent novel amplicon associated with poor prognosis.
IHC / Human
Back signaling by the Nrg-1 intracellular domain.
ICC/IF / Mouse
Reciprocal signaling between spiral ganglion neurons and Schwann cells involves neuregulin and neurotrophins.
ICC/IF / Rat
