ARG63739
anti-NPM1 / Nucleophosmin antibody
anti-NPM1 / Nucleophosmin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Nucleolar Marker antibody; GC Marker antibody; Granular Component Marker antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes NPM1 / Nucleophosmin |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Cow, Rat, Dog |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | This antibody is expected to recognise both report isoforms (NP_002511.1; NP_954654.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NPM1 / Nucleophosmin |
| Antigen Species | Human |
| Immunogen | C-QEAIQDLWQWRKSL |
| Conjugation | Un-conjugated |
| Alternate Names | NPM; Nucleolar protein NO38; B23; Nucleophosmin; Numatrin; Nucleolar phosphoprotein B23 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Background | This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009] |
| Research Area | Nucleolar Marker antibody; GC Marker antibody; Granular Component Marker antibody |
| Calculated MW | 33 kDa |
| PTM | Acetylated at C-terminal lysine residues, thereby increasing affinity to histones. ADP-ribosylated. Phosphorylated at Ser-4 by PLK1 and PLK2. Phosphorylation at Ser-4 by PLK2 in S phase is required for centriole duplication and is sufficient to trigger centriole replication. Phosphorylation at Ser-4 by PLK1 takes place during mitosis. Phosphorylated by CDK2 at Ser-125 and Thr-199. Phosphorylation at Thr-199 may trigger initiation of centrosome duplication. Phosphorylated by CDK1 at Thr-199, Thr-219, Thr-234 and Thr-237 during cell mitosis. When these four sites are phosphorated, RNA-binding activity seem to be abolished. May be phosphorylated at Ser-70 by NEK2. The Thr-199 phosphorylated form has higher affinity for ROCK2. CDK6 triggers Thr-199 phosphorylation when complexed to Kaposi's sarcoma herpesvirus (KSHV) V-cyclin, leading to viral reactivation by reducing viral LANA levels. Sumoylated by ARF. May be ubiquitinated. Ubiquitination leads to proteasomal degradation. |
Images (4) Click the Picture to Zoom In
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ARG63739 anti-NPM1 / Nucleophosmin antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG63739 anti-NPM1 / Nucleophosmin antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63739 anti-NPM1 / Nucleophosmin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63739 anti-NPM1 / Nucleophosmin antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63739 anti-NPM1 / Nucleophosmin antibody WB image
Western blot: 35 µg of NIH/3T3 (A), Daudi (B), Jurkat (C) and K562 (D) cell lysates (in RIPA buffer) stained with ARG63739 anti-NPM1 / Nucleophosmin antibody at 1 µg/ml (A), 0.1 µg/ml (B, C) and 0.01 µg/ml (D) dilutions and incubated at RT for 1 hour.
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ARG63739 anti-NPM1 / Nucleophosmin antibody WB image
Western blot: 35 µg of Mouse spleen lysate (in RIPA buffer) stained with ARG63739 anti-NPM1 / Nucleophosmin antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
