ARG64944
anti-NOX2 / gp91phox antibody
anti-NOX2 / gp91phox antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Cancer antibody; Immune System antibody; Metabolism antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes NOX2 / gp91phox |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Dog |
| Tested Application | IHC-P |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NOX2 / gp91phox |
| Antigen Species | Human |
| Immunogen | C-SYLNFARKRIKNP |
| Conjugation | Un-conjugated |
| Alternate Names | Neutrophil cytochrome b 91 kDa polypeptide; gp91-phox; NADPH oxidase 2; Heme-binding membrane glycoprotein gp91phox; 558; CGD91-phox; gp91-1; EC 1.-.-.-; Cytochrome b; GP91-1; IMD34; CGD; GP91-PHOX; AMCBX2; GP91PHOX; NOX2; Cytochrome b-245 heavy chain; p22 phagocyte B-cytochrome; p91-PHOX; Superoxide-generating NADPH oxidase heavy chain subunit; Cytochrome b558 subunit beta |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008] |
| Research Area | Cancer antibody; Immune System antibody; Metabolism antibody |
| Calculated MW | 65 kDa |
| PTM | Glycosylated. Phosphorylated on Ser and Thr residues. |
Images (1) Click the Picture to Zoom In
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ARG64944 anti-NOX2 / gp91phox antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64944 anti-NOX2 / gp91phox antibody at 3.8 µg/ml dilution followed by AP-staining.
