ARG44107

anti-NOP2 antibody

anti-NOP2 antibody for Flow cytometry,ICC/IF,Western blot and Human

Overview

Product Description Rabbit Polyclonal recognizes NOP2
Tested Reactivity Hu
Tested Application FACS, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NOP2
Antigen Species Human
Immunogen Human NOP2 recombinant protein (Position: L210-Q747).
Conjugation Un-conjugated
Alternate Names NOP2; NOP2 Nucleolar Protein; NSUN1; NOP120; P120; NOL1; Probable 28S RRNA (Cytosine(4447)-C(5))-Methyltransferase; Proliferation-Associated Nucleolar Protein P120; Proliferating-Cell Nucleolar Antigen P120; Nucleolar Protein 1, 120kDa; Nucleolar Protein 2 Homolog; Putative Ribosomal RNA Methyltransferase NOP2; NOP2 Nucleolar Protein Homolog (Yeast); NOL1/NOP2/Sun Domain Family, Member 1; Nucleolar Protein 2 Homolog (Yeast); NOP2/Sun Domain Family, Member 1; NOP2 Nucleolar Protein Homolog; Nucleolar Protein 1 (120kD); Nucleolar Protein 1; EC 2.1.1.-; EC 2.1.1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
ICC/IF5 µg/ml
WB0.1 - 0.25 µg/ml
Application Note The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4839 Human NOP2

Swiss-port # P46087 Human Probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase

Gene Symbol NOP2
Gene Full Name NOP2 Nucleolar Protein
Background Enables RNA binding activity. Involved in positive regulation of cell population proliferation; regulation of signal transduction by p53 class mediator; and ribosomal large subunit assembly. Located in nucleolus.
Function Involved in ribosomal large subunit assembly.
Cellular Localization Nucleus
Calculated MW 89 kDa
PTM Acetylation, Citrullination, Isopeptide bond, Phosphoprotein, Ubl conjugation

Images (3) Click the Picture to Zoom In

  • ARG44107 anti-NOP2 antibody ICC/IF image

    Immunofluorescence: U2OS stained with ARG44107 anti-NOP2 antibody at 5 μg/ml dilution.

  • ARG44107 anti-NOP2 antibody WB image

    Western blot: A549 and HeLa stained with ARG44107 anti-NOP2 antibody at 0.5 μg/mL dilution.

  • ARG44107 anti-NOP2 antibody FACS image

    Flow Cytometry: RT4 stained with ARG44107 anti-NOP2 antibody at 1 µg/10^6 cells dilution.