ARG63972
anti-NONO / p54nrb antibody
anti-NONO / p54nrb antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes NONO / p54nrb |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Dog, Rat |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NONO / p54nrb |
| Antigen Species | Human |
| Immunogen | C-NRAAPGAEFAPNK |
| Conjugation | Un-conjugated |
| Alternate Names | Non-POU domain-containing octamer-binding protein; p54nrb; NonO protein; DNA-binding p52/p100 complex, 52 kDa subunit; P54NRB; NRB54; p54; NMT55; 55 kDa nuclear protein; 54 kDa nuclear RNA- and DNA-binding protein; P54; PPP1R114; nrb |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q15233 Human Non-POU domain-containing octamer-binding protein Swiss-port # Q99K48 Mouse Non-POU domain-containing octamer-binding protein |
|---|---|
| Background | This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 54 kDa |
| PTM | The N-terminus is blocked. |
Images (4) Click the Picture to Zoom In
-
ARG63972 anti-NONO / p54nrb antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human spleen (Steamed antigen retrieval with citrate buffer pH 6.0) stained with ARG63972 anti-NONO / p54nrb antibody at 3.8 µg/ml dilution, followed by AP-staining.
-
ARG63972 anti-NONO / p54nrb antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63972 anti-NONO / p54nrb antibody at 3.75 µg/ml dilution followed by AP-staining.
-
ARG63972 anti-NONO / p54nrb antibody WB image
Western blot: 35 µg of Mouse brain lysate stained with ARG63972 anti-NONO / p54nrb antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
-
ARG63972 anti-NONO / p54nrb antibody WB image
Western blot: 1) Mock transfection and 2) Human NONO expressing plasmid transfected HEK293 cell lysate stained with ARG63972 anti-NONO / p54nrb antibody.
