ARG40320

anti-NOB1 antibody

anti-NOB1 antibody for ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes NOB1
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NOB1
Antigen Species Human
Immunogen Recombinant fusion protein of Human NOB1.
Conjugation Un-conjugated
Alternate Names Phosphorylation regulatory protein HP-10; RNA-binding protein NOB1; Protein ART-4; MST158; MSTP158; NOB1P; ART-4; PSMD8BP1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control BxPC-3
Observed Size 47 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 28987 Human NOB1

GeneID: 291996 Rat NOB1

GeneID: 67619 Mouse NOB1

Gene Symbol NOB1
Gene Full Name NIN1/RPN12 binding protein 1 homolog
Background In yeast, over 200 protein and RNA cofactors are required for ribosome assembly, and these are generally conserved in eukaryotes. These factors orchestrate modification and cleavage of the initial 35S precursor rRNA transcript into the mature 18S, 5.8S, and 25S rRNAs, folding of the rRNA, and binding of ribosomal proteins and 5S RNA. Nob1 is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA (Lamanna and Karbstein, 2009 [PubMed 19706509]).[supplied by OMIM, Nov 2010]
Function May play a role in mRNA degradation. [UniProt]
Cellular Localization Nucleus. [UniProt]
Calculated MW 47 kDa

Images (1) Click the Picture to Zoom In

  • ARG40320 anti-NOB1 antibody WB image

    Western blot: 25 µg of BxPC-3 cell lysate stained with ARG40320 anti-NOB1 antibody at 1:1000 dilution.