ARG57060

anti-NNMT antibody [11G11]

anti-NNMT antibody [11G11] for Flow cytometry,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [11G11] recognizes NNMT
Tested Reactivity Hu
Tested Application FACS, WB
Host Mouse
Clonality Monoclonal
Clone 11G11
Isotype IgG2a, kappa
Target Name NNMT
Antigen Species Human
Immunogen Recombinant fragment around aa. 1-264 of Human NNMT.
Conjugation Un-conjugated
Alternate Names Nicotinamide N-methyltransferase; EC 2.1.1.1

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 10% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4837 Human NNMT

Swiss-port # P40261 Human Nicotinamide N-methyltransferase

Gene Symbol NNMT
Gene Full Name nicotinamide N-methyltransferase
Background N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq, Jul 2008]
Function Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. [UniProt]
Calculated MW 30 kDa

Images (2) Click the Picture to Zoom In

  • ARG57060 anti-NNMT antibody [11G11] FACS image

    Flow Cytometry: HeLa cell line stained with ARG57060 anti-NNMT antibody [11G11] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).

  • ARG57060 anti-NNMT antibody [11G11] WB image

    Western blot: 40 µg of 1) HeLa cell lysate, 2) A549 cell lysate stained with ARG57060 anti-NNMT antibody [11G11] at 1:1000.