ARG52372
anti-NMDAR2C phospho (Ser1096) antibody
anti-NMDAR2C phospho (Ser1096) antibody for Western blot and Mouse,Rat
Neuroscience antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NMDAR2C phospho (Ser1096) |
|---|---|
| Tested Reactivity | Ms, Rat |
| Predict Reactivity | Hu, Bov, Dog, NHuPrm |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NMDAR2C |
| Antigen Species | Rat |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser1096 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | Glutamate receptor ionotropic, NMDA 2C; NR2C; Glutamate [NMDA] receptor subunit epsilon-3; GluN2C; N-methyl D-aspartate receptor subtype 2C; NMDAR2C |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~140k NR2C subunit of the NMDA receptor phosphorylated at Ser1096. Immunolabeling is blocked by preadsorption of antibody with the phospho-peptide that was used to geneRate the antibody but not by the corresponding dephospho-peptide. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q00961 Rat Glutamate receptor ionotropic, NMDA 2C Swiss-port # Q01098 Mouse Glutamate receptor ionotropic, NMDA 2C |
|---|---|
| Gene Symbol | GRIN2C |
| Gene Full Name | glutamate receptor, ionotropic, N-methyl D-aspartate 2C |
| Background | The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The NR2C subunit of the receptor is thought to influence the NMDAR conductance level (Ebralidze et al., 1996). Phosphorylation of Ser1096 by PKB on NR2C has been recently demonstrated to regulate NMDA receptor binding to 14-3-3 (Chen & Roche 2009). |
| Research Area | Neuroscience antibody |
| Calculated MW | 134 kDa |
Images (1) Click the Picture to Zoom In
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ARG52372 anti-NMDAR2C phospho (Ser1096) antibody WB image
Western blot: Mouse brain lysate stained with ARG52372 anti-NMDAR2C phospho (Ser1096) antibody showing specific immunolabeling of the ~140k NR2C subunit of the NMDA receptor phosphorylated at Ser1096. The phosphospecificity is shown in the second lane where immunoreactivity is blocked by preadsorption with the phospho-peptide (Peptide) used as antigen but not by the dephosphopeptide (not shown).
