ARG51666
anti-NMDAR2B phospho (Tyr1474) antibody
anti-NMDAR2B phospho (Tyr1474) antibody for ICC/IF,Western blot and Human,Mouse,Rat
Neuroscience antibody; Postsynaptic Receptor antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NMDAR2B phospho (Tyr1474) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NMDAR2B |
| Antigen Species | Human |
| Immunogen | Peptide sequence around phosphorylation site of Tyr1474 (H-V-Y(p)-E-K) derived from Human NMDAR2B. |
| Conjugation | Un-conjugated |
| Alternate Names | MRD6; EIEE27; NR2B; hNR3; GluN2B; NR3; N-methyl D-aspartate receptor subtype 2B; Glutamate receptor ionotropic, NMDA 2B; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl-D-aspartate receptor subunit 3; NMDAR2B |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GRIN2B |
| Gene Full Name | glutamate receptor, ionotropic, N-methyl D-aspartate 2B |
| Background | NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. |
| Function | NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death (By similarity). [UniProt] |
| Research Area | Neuroscience antibody; Postsynaptic Receptor antibody |
| Calculated MW | 166 kDa |
| PTM | Phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity. |
Images (2) Click the Picture to Zoom In
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ARG51666 anti-NMDAR2B phospho (Tyr1474) antibody WB image
Western blot: Extracts from A431 cells untreated or treated with EGF stained with ARG51666 anti-NMDAR2B phospho (Tyr1474) antibody.
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ARG51666 anti-NMDAR2B phospho (Tyr1474) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with ARG51666 anti-NMDAR2B phospho (Tyr1474) antibody.
