ARG22230

anti-NMDAR2B antibody [S59-36]

anti-NMDAR2B antibody [S59-36] for ICC/IF,Immunohistochemistry,IHC-Whole mount,Immunoprecipitation,Western blot and Human,Mouse,Rat,D. melanogaster

Neuroscience antibody; Postsynaptic Receptor antibody
publication_link Publication1

Overview

Product Description Mouse Monoclonal antibody [S59-36] recognizes NMDAR2B
Tested Reactivity Hu, Ms, Rat, Dm
Tested Application ICC/IF, IHC, IHC-Wmt, IP, WB
Specificity Detects ~166kDa. No cross-reactivity against NR2A.
Host Mouse
Clonality Monoclonal
Clone S59-36
Isotype IgG2b
Target Name NMDAR2B
Antigen Species Rat
Immunogen Fusion protein around aa. 20-271 (extracellular N-terminus) of Rat NMDAR2B
Conjugation Un-conjugated
Alternate Names MRD6; EIEE27; NR2B; hNR3; GluN2B; NR3; N-methyl D-aspartate receptor subtype 2B; Glutamate receptor ionotropic, NMDA 2B; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl-D-aspartate receptor subunit 3; NMDAR2B

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100
IHC1:1000
IHC-WmtAssay-dependent
IPAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14812 Mouse GRIN2B

GeneID: 24410 Rat GRIN2B

GeneID: 2904 Human GRIN2B

Gene Symbol Grin2b
Gene Full Name glutamate receptor, ionotropic, N-methyl D-aspartate 2B
Background N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain. [provided by RefSeq, Jul 2008]
Function NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death (By similarity). [UniProt]
Cellular Localization Cell Junction, Cell membrane, postsynaptic cell membrane, Synapse
Highlight Related products:
anti-NMDAR2B antibody [S59-36]
Research Area Neuroscience antibody; Postsynaptic Receptor antibody
Calculated MW 166 kDa
PTM Phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity.

Images (2) Click the Picture to Zoom In

  • ARG22230 anti-NMDAR2B antibody [S59-36] WB image

    Western blot: Rat brain membrane lysate stained with ARG22230 anti-NMDAR2B antibody [S59-36] at 1:1000 dilution.

  • ARG22230 anti-NMDAR2B antibody [S59-36] WB image

    Western blot: 20 µl of NR2B HEK-T lysates stained with ARG22230 anti-NMDAR2B antibody [S59-36] at 1:250 dilution (TBS for 60 min at RT). Block: 5% milk + TBST 1 hr at RT.

Specific References

Myc supercompetitor cells exploit the NMDA receptor to subdue their wild-type neighbours via cell competition.

IHC-Wmt / Drosophila

Agnes R. Banreti et al.
Preprint.,  (2020)

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