ARG52352
anti-NMDAR1 antibody [R1JHL]
anti-NMDAR1 antibody [R1JHL] for Immunoprecipitation,Western blot and Mouse,Rat
Neuroscience antibody
Overview
| Product Description | Mouse Monoclonal antibody [R1JHL] recognizes NMDAR1 |
|---|---|
| Tested Reactivity | Ms, Rat |
| Tested Application | IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | R1JHL |
| Isotype | IgG |
| Target Name | NMDAR1 |
| Antigen Species | Rat |
| Immunogen | Fusion protein containing amino acids 1-564 of the NR1 subunit |
| Conjugation | Un-conjugated |
| Alternate Names | NMDA1; GluN1; MRD8; NMD-R1; Glutamate receptor ionotropic, NMDA 1; Glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; NR1; NMDAR1 |
Application Instructions
| Application Note | Specific for the ~120k NR1 subunit of the NMDA receptor. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Properties
| Form | Liquid |
|---|---|
| Purification | Culture supernatant |
| Buffer | PBS (pH 7.4), 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl and 1.5 mM KH2PO4. |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P35438 Mouse Glutamate receptor ionotropic, NMDA 1 Swiss-port # P35439 Rat Glutamate receptor ionotropic, NMDA 1 |
|---|---|
| Gene Symbol | Grin1 |
| Gene Full Name | glutamate receptor, ionotropic, N-methyl D-aspartate 1 |
| Background | The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008] |
| Function | NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors. [UniProt] |
| Research Area | Neuroscience antibody |
| Calculated MW | 105 kDa |
| PTM | NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity. |
Images (1) Click the Picture to Zoom In
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ARG52352 anti-NMDAR1 antibody [R1JHL] WB image
Western Blot: 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~120k NR1 subunit of the NMDA receptor stained with ARG52352 NMDAR1 antibody [R1JHL]
Clone References
The human immunodeficiency virus coat protein gp120 promotes forward trafficking and surface clustering of NMDA receptors in membrane microdomains.
ICC/IF / Rat
Reduced cortical BDNF expression and aberrant memory in Carf knock-out mice.
WB / Mouse
A neuronal role for SNAP-23 in postsynaptic glutamate receptor trafficking.
WB / Rat
Prion protein expression modulates neuronal copper content.
WB / Mouse
