ARG63998
anti-NMDAR1 antibody
anti-NMDAR1 antibody for Western blot and Human,Rat
Neuroscience antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes NMDAR1 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Predict Reactivity | Ms, Dog |
| Tested Application | WB |
| Specificity | This antibody is expected to recognise all three reported isoforms (NP_000823.4; NP_067544.1; NP_015566.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NMDAR1 |
| Antigen Species | Human |
| Immunogen | C-TQERVNNSNKKE |
| Conjugation | Un-conjugated |
| Alternate Names | NMDA1; GluN1; MRD8; NMD-R1; Glutamate receptor ionotropic, NMDA 1; Glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; NR1; NMDAR1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P35439 Rat Glutamate receptor ionotropic, NMDA 1 Swiss-port # Q05586 Human Glutamate receptor ionotropic, NMDA 1 |
|---|---|
| Background | The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008] |
| Research Area | Neuroscience antibody |
| Calculated MW | 105 kDa |
| PTM | NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity. |
Images (1) Click the Picture to Zoom In
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ARG63998 anti-NMDAR1 antibody WB image
Western blot: 35 µg of Human amygdala (A) and Rat brain (B) lysates (in RIPA buffer) stained with ARG63998 anti-NMDAR1 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
