ARG52366
anti-NMDA NR2B Subunit phospho (Ser1166) antibody
anti-NMDA NR2B Subunit phospho (Ser1166) antibody for Western blot and Mouse,Rat
Neuroscience antibody; Postsynaptic Receptor antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NMDA NR2B Subunit phospho (Ser1166) |
|---|---|
| Tested Reactivity | Ms, Rat |
| Predict Reactivity | Hu, Dog, NHuPrm, Xenopus laevis |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NMDA NR2B Subunit |
| Antigen Species | Rat |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser1166 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | MRD6; EIEE27; NR2B; hNR3; GluN2B; NR3; N-methyl D-aspartate receptor subtype 2B; Glutamate receptor ionotropic, NMDA 2B; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl-D-aspartate receptor subunit 3; NMDAR2B |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | Specific for the ~180k NMDAR NR2B subunit phosphorylated at Ser1166. Immunolabeling of the NMDA NR2B subunit band is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q00960 Rat Glutamate receptor ionotropic, NMDA 2B Swiss-port # Q01097 Mouse Glutamate receptor ionotropic, NMDA 2B |
|---|---|
| Gene Symbol | GRIN2B |
| Gene Full Name | glutamate receptor, ionotropic, N-methyl D-aspartate 2B |
| Background | The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). Overexpression of the NR2B-subunit of the NMDA Receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a; Clayton et al., 2002b). Phosphorylation of Ser1166 is thought to play an essential role in memory and neuronal development. |
| Research Area | Neuroscience antibody; Postsynaptic Receptor antibody |
| Calculated MW | 166 kDa |
| PTM | Phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity. |
Images (1) Click the Picture to Zoom In
-
ARG52366 anti-NMDA NR2B Subunit phospho (Ser1166) antibody WB image
Western blot: Rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Ser 1166 (Control) stained with ARG52366 anti-NMDA NR2B Subunit phospho (Ser1166) antibody.
Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide).
.
