ARG57180
anti-NM23A antibody [5F4]
anti-NM23A antibody [5F4] for Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [5F4] recognizes NM23A |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 5F4 |
| Isotype | IgG2b, kappa |
| Target Name | NM23A |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-152 of Human NM23A |
| Conjugation | Un-conjugated |
| Alternate Names | NDP kinase A; NDPK-A; NM23; Nucleoside diphosphate kinase A; Granzyme A-activated DNase; NDKA; NBS; NM23-H1; NB; NDPKA; NDK A; Tumor metastatic process-associated protein; EC 2.7.4.6; GAAD; AWD; Metastasis inhibition factor nm23 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NME1 |
| Gene Full Name | NME/NM23 nucleoside diphosphate kinase 1 |
| Background | This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008] |
| Function | Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair. [UniProt] |
| Calculated MW | 17 kDa |
Images (2) Click the Picture to Zoom In
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ARG57180 anti-NM23A antibody [5F4] WB image
Western blot: 40 µg of 1) HeLa, 2) A549, 3) Jurkat, 4) HepG2, 5) MCF7, and 6) PC3 cell lysates stained with ARG57180 anti-NM23A antibody [5F4] at 1:1000.
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ARG57180 anti-NM23A antibody [5F4] WB image
Western blot: 50 ng of 1) NME1, 2) NME2, 3) NME3, and 4) NME4 recombinant proteins stained with ARG57180 anti-NM23A antibody [5F4] at 1:1000.
