ARG59418

anti-NLRC4 / CARD12 antibody

anti-NLRC4 / CARD12 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

NLRC4 Inflammasome Study antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes NLRC4 / CARD12
Tested Reactivity Hu, Ms
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NLRC4 / CARD12
Antigen Species Mouse
Immunogen Recombinant fusion protein corresponding to aa. 89-159 of Mouse NLRC4 (NP_001028539.1).
Conjugation Un-conjugated
Alternate Names CLAN; AIFEC; IPAF; CLANA; CARD12; CLANC; CLANB; CLAND; NLR family CARD domain-containing protein 4; Caspase recruitment domain-containing protein 12; Ipaf; CLR2.1; CARD, LRR, and NACHT-containing protein; CLAN1; Ice protease-activating factor; Clan protein; FCAS4

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse spleen and U87
Observed Size 116 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 268973 Mouse NLRC4

GeneID: 58484 Human NLRC4

Swiss-port # Q3UP24 Mouse NLR family CARD domain-containing protein 4

Swiss-port # Q9NPP4 Human NLR family CARD domain-containing protein 4

Gene Symbol NLRC4
Gene Full Name NLR family, CARD domain containing 4
Background NLRC4 / CARD12 is a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
Function NLRC4 / CARD12 is a key component. of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed:15107016). The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria. [UniProt]
Cellular Localization Cytoplasm. Cytoplasm, cytosol. Note=Cytoplasmic filaments. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30332 NLRC4 Inflammasome Antibody Panel
Related products:
NLRC4 antibodies; NLRC4 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Exploring Antiviral Immune Response
Research Area NLRC4 Inflammasome Study antibody
Calculated MW 116 kDa
PTM Phosphorylated at Ser-533 following infection of macrophages with S.typhimurium (Salmonella). Phosphorylation is essential for NLRC4 inflammasome function to promote caspase-1 activation and pyroptosis. PRKCD phosphorylates Ser-533 in vitro (By similarity). [UniProt]

Images (6) Click the Picture to Zoom In

  • ARG59418 anti-NLRC4 / CARD12 antibody ICC/IF image

    Immunofluorescence: THP-1 cells stained with ARG59418 anti-NLRC4 / CARD12 antibody (red).

  • ARG59418 anti-NLRC4 / CARD12 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human lung stained with ARG59418 anti-NLRC4 / CARD12 antibody at 1:100 dilution (40x lens).

  • ARG59418 anti-NLRC4 / CARD12 antibody WB image

    Western blot: 25 µg of Mouse spleen and U87 cell lysate stained with ARG59418 anti-NLRC4 / CARD12 antibody at 1:3000 dilution.

  • ARG59418 anti-NLRC4 / CARD12 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human colon stained with ARG59418 anti-NLRC4 / CARD12 antibody at 1:100 dilution (40x lens).

  • ARG59418 anti-NLRC4 / CARD12 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human gastric stained with ARG59418 anti-NLRC4 / CARD12 antibody at 1:100 dilution (40x lens).

  • ARG59418 anti-NLRC4 / CARD12 antibody IHC-P image

    Immunohistochemistry: Mouse lung stained with ARG59418 anti-NLRC4 / CARD12 antibody (orange-red).