Overview

Product Description	Mouse Monoclonal antibody recognizes NLK
Tested Reactivity	Hu
Tested Application	WB
Host	Mouse
Clonality	Monoclonal
Clone	1146CT24.2.1
Isotype	IgG2a
Target Name	NLK
Antigen Species	Human
Immunogen	Purified His-tagged NLK protein
Conjugation	Un-conjugated
Alternate Names	Protein LAK1; EC 2.7.11.24; Nemo-like kinase; Serine/threonine-protein kinase NLK

Application Instructions

Application Suggestion	Tested Application Dilution WB 1:1000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	HeLa

Properties

Form	Liquid
Purification	Purification with Protein G.
Buffer	PBS and 0.09% (W/V) Sodium azide
Preservative	0.09% (W/V) Sodium azide
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 51701 Human NLK Swiss-port # Q9UBE8 Human Serine/threonine-protein kinase NLK
Gene Symbol	NLK
Gene Full Name	nemo-like kinase
Function	Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1. [UniProt]
Cellular Localization	Nucleus. Cytoplasm. Note=Predominantly nuclear. A smaller fraction is cytoplasmic (By similarity).
Calculated MW	58 kDa
PTM	Phosphorylated on Thr-298. Intermolecular autophosphorylation on Thr-298 activates the enzyme.

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