ARG44061
anti-NHP2 antibody
anti-NHP2 antibody for Flow cytometry,ICC/IF,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal recognizes NHP2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NHP2 |
| Antigen Species | Human |
| Immunogen | Human NHP2 recombinant protein (Position: E21-Q136). |
| Conjugation | Un-conjugated |
| Alternate Names | NHP2P; H/ACA ribonucleoprotein complex subunit 2; Nucleolar protein family A member 2; NOLA2; DKCB2; snoRNP protein NHP2 |
Application Instructions
| Application Suggestion |
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| Application Note | The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9NX24 Human H/ACA ribonucleoprotein complex subunit 2 |
|---|---|
| Gene Symbol | NHP2 |
| Gene Full Name | NHP2 ribonucleoprotein |
| Background | This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA1 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. The four H/ACA snoRNP proteins are also components of the telomerase complex. This gene encodes a protein related to Saccharomyces cerevisiae Nhp2p. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008] |
| Function | Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ("psi") residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. [UniProt] |
| Cellular Localization | Nucleus, nucleolus. Nucleus, Cajal body. Note=Also localized to Cajal bodies (coiled bodies). [UniProt] |
| Calculated MW | 17 kDa |
Images (3) Click the Picture to Zoom In
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ARG44061 anti-NHP2 antibody ICC/IF image
Immunofluorescence: A549 stained with ARG44061 anti-NHP2 antibody at 5 μg/mL dilution.
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ARG44061 anti-NHP2 antibody WB image
Western blot: SH-SY5Y, Caco-2 and Hela stained with ARG44061 anti-NHP2 antibody at 0.5 μg/mL dilution.
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ARG44061 anti-NHP2 antibody FACS image
Flow Cytometry: SH-SY5Y stained with ARG44061 anti-NHP2 antibody at 1 µg/1x10^6 cells dilution.
