Overview

Product Description	Rabbit Polyclonal antibody recognizes NFYB
Tested Reactivity	Hu
Tested Application	WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	NFYB
Antigen Species	Human
Immunogen	Synthetic peptide derived from the N-terminal region of Human NF-YB. at AA rangle: 10-90
Conjugation	Un-conjugated
Alternate Names	NF-YB; HAP3; Nuclear transcription factor Y subunit B; CBF-A; CAAT box DNA-binding protein subunit B; CBF-B; Nuclear transcription factor Y subunit beta

Application Instructions

Application Suggestion	Tested Application Dilution WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification	Affinity purification with immunogen.
Buffer	PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol and 0.5% BSA
Concentration	1 mg/ml
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 4801 Human NFYB Swiss-port # P25208 Human Nuclear transcription factor Y subunit beta
Gene Symbol	NFYB
Gene Full Name	nuclear transcription factor Y, beta
Background	The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008]
Function	Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. [UniProt]
Cellular Localization	Nucleus. [UniProt]
Calculated MW	23 kDa
PTM	Monoubiquitination at Lys-140 plays an important role in transcriptional activation by allowing the deposition of histone H3 methylations as well as histone H2B monoubiquitination at 'Lys-121'. [UniProt]

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