ARG63152
anti-NFIL3 antibody
anti-NFIL3 antibody for Flow cytometry,Western blot and Human
Gene Regulation antibody; Immune System antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes NFIL3 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat |
| Tested Application | FACS, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NFIL3 |
| Antigen Species | Human |
| Immunogen | C-RLIATQPISASDSG |
| Conjugation | Un-conjugated |
| Alternate Names | Nuclear factor interleukin-3-regulated protein; NF-IL3A; E4BP4; IL3BP1; Transcriptional activator NF-IL3A; NFIL3A; Interleukin-3 promoter transcriptional activator; E4 promoter-binding protein 4; Interleukin-3-binding protein 1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q16649 Human Nuclear factor interleukin-3-regulated protein |
|---|---|
| Background | Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells, natural killer (NK) cells, and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors, such as NFIL3, that bind to regulatory regions of the gene, usually upstream of the transcription start site (Zhang et al., 1995 [PubMed 7565758]).[supplied by OMIM, Feb 2009] |
| Research Area | Gene Regulation antibody; Immune System antibody |
| Calculated MW | 51 kDa |
Images (2) Click the Picture to Zoom In
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ARG63152 anti-NFIL3 antibody WB image
Western Blot: HepG2 lysate (RIPA buffer, 20 µg total protein per lane) stained with ARG63152 anti-NFIL3 antibody at 0.5 µg/ml dilution.
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ARG63152 anti-NFIL3 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed Human peripheral blood mononuclear cells permeabilized with 0.5% Triton. Cells were stained with ARG63152 anti-NFIL3 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
