ARG41147

anti-NF66 / alpha Internexin antibody

anti-NF66 / alpha Internexin antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes NF66 / alpha Internexin
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NF66 / alpha Internexin
Antigen Species Human
Immunogen Synthetic peptide derived from Human alpha Internexin.
Conjugation Un-conjugated
Alternate Names Neurofilament 5; Neurofilament-66; Alpha-Inx; NEF5; NF-66; Alpha-internexin; 66 kDa neurofilament protein; TXBP-1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:20
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
IP1:50
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control SH-SY5Y

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 226180 Mouse INA

GeneID: 24503 Rat INA

GeneID: 9118 Human INA

Gene Symbol INA
Gene Full Name internexin neuronal intermediate filament protein, alpha
Background Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene is a member of the intermediate filament family and is involved in the morphogenesis of neurons. [provided by RefSeq, Jun 2009]
Function Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges. [UniProt]
Calculated MW 55 kDa
PTM O-glycosylated. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG41147 anti-NF66 / alpha Internexin antibody WB image

    Western blot: SH-SY5Y cell lysate stained with ARG41147 anti-NF66 / alpha Internexin antibody.