ARG51750
anti-NF2 / Merlin phospho (Ser518) antibody
anti-NF2 / Merlin phospho (Ser518) antibody for ICC/IF,Western blot and Human,Mouse,Rat
Cancer antibody; Gene Regulation antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NF2 / Merlin phospho (Ser518) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NF2 / Merlin |
| Antigen Species | Human |
| Immunogen | Peptide sequence around phosphorylation site of serine 518 (R-L-S(p)-M-E) derived from Human Merlin. |
| Conjugation | Un-conjugated |
| Alternate Names | Merlin; Schwannomin; SCH; Schwannomerlin; ACN; Moesin-ezrin-radixin-like protein; BANF; Neurofibromin-2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NF2 |
| Gene Full Name | neurofibromin 2 (merlin) |
| Background | Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. |
| Function | Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex. [UniProt] |
| Research Area | Cancer antibody; Gene Regulation antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 70 kDa |
| PTM | Phosphorylation of Ser-518 inhibits nuclear localization by disrupting the intramolecular association of the FERM domain with the C-terminal tail (PubMed:20178741). The dephosphorylation of Ser-518 favors the interaction with NOP53 (PubMed:21167305). Ubiquitinated by the CUL4A-RBX1-DDB1-DCAF1/VprBP E3 ubiquitin-protein ligase complex for ubiquitination and subsequent proteasome-dependent degradation. |
Images (3) Click the Picture to Zoom In
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ARG51750 anti-NF2 / Merlin phospho (Ser518) antibody WB image
Western blot: Extracts from HUVEC cells untreated(lane 1) or treated with IFN-a(lane 2) stained with ARG51750 anti-NF2 / Merlin phospho (Ser518) antibody.
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ARG51750 anti-NF2 / Merlin phospho (Ser518) antibody WB image
Western blot: Extracts from C6 cells, treated with calf intestinal phosphatase (CIP), stained with ARG51750 anti-NF2 / Merlin phospho (Ser518) antibody.
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ARG51750 anti-NF2 / Merlin phospho (Ser518) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with ARG51750 anti-NF2 / Merlin phospho (Ser518) antibody.
