ARG40442
anti-NEU1 / Neuraminidase antibody
anti-NEU1 / Neuraminidase antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NEU1 / Neuraminidase |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NEU1 / Neuraminidase |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 156-415 of Human NEU1 (NP_000425.1). |
| Conjugation | Un-conjugated |
| Alternate Names | NANH; Acetylneuraminyl hydrolase; SIAL1; Lysosomal sialidase; EC 3.2.1.18; N-acetyl-alpha-neuraminidase 1; Sialidase-1; NEU; G9 sialidase |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Mouse liver | ||||||
| Observed Size | 48 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NEU1 |
| Gene Full Name | sialidase 1 (lysosomal sialidase) |
| Background | The protein encoded by this gene is a lysosomal enzyme that cleaves terminal sialic acid residues from substrates such as glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (the latter is also referred to as 'protective protein'). Mutations in this gene can lead to sialidosis, a lysosomal storage disease that can be type 1 (cherry red spot-myoclonus syndrome or normosomatic type), which is late-onset, or type 2 (the dysmorphic type), which occurs at an earlier age with increased severity. [provided by RefSeq, Jul 2008] |
| Function | Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage. [UniProt] |
| Cellular Localization | Lysosome membrane; Peripheral membrane protein; Lumenal side. Lysosome lumen. Cell membrane. Cytoplasmic vesicle. Lysosome. Note=Localized not only on the inner side of the lysosomal membrane and in the lysosomal lumen, but also on the plasma membrane and in intracellular vesicles. [UniProt] |
| Calculated MW | 45 kDa |
| PTM | N-glycosylated. Phosphorylation of tyrosine within the internalization signal results in inhibition of sialidase internalization and blockage on the plasma membrane. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG40442 anti-NEU1 / Neuraminidase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse brain stained with ARG40442 anti-NEU1 / Neuraminidase antibody at 1:100 dilution.
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ARG40442 anti-NEU1 / Neuraminidase antibody WB image
Western blot: 25 µg of Mouse liver lysate stained with ARG40442 anti-NEU1 / Neuraminidase antibody at 1:1000 dilution.
