ARG64078
anti-NEDD1 antibody
anti-NEDD1 antibody for Western blot and Human
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes NEDD1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NEDD1 |
| Antigen Species | Human |
| Immunogen | C-NQTRNSEKFEKPEN |
| Conjugation | Un-conjugated |
| Alternate Names | GCP-WD; Protein NEDD1; NEDD-1; Neural precursor cell expressed developmentally down-regulated protein 1; TUBGCP7 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NEDD1 |
| Gene Full Name | neural precursor cell expressed, developmentally down-regulated 1 |
| Function | Required for mitosis progression. Promotes the nucleation of microtubules from the spindle. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody |
| Calculated MW | 72 kDa |
| PTM | During mitosis, prior phosphorylation on Thr-550 by CDK1 promotes subsequent phosphorylation by PLK1 on Thr-382, Ser-397, Ser-426 and Ser-637. Phosphorylated NEDD1 can interact with gamma-tubulin for targeting the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation. |
Images (1) Click the Picture to Zoom In
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ARG64078 anti-NEDD1 antibody WB image
Western Blot: HeLa cell lysate (35 µg protein in RIPA buffer) stained with ARG64078 anti-NEDD1 antibody at 0.1 µg/ml dilution.
