ARG65578
anti-NDUFS8 antibody
anti-NDUFS8 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NDUFS8 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NDUFS8 |
| Antigen Species | Human |
| Immunogen | Fusion protein of human NDUFS8 |
| Conjugation | Un-conjugated |
| Alternate Names | EC 1.6.5.3; TYKY; NADH-ubiquinone oxidoreductase 23 kDa subunit; CI-23k; NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial; CI23KD; Complex I-23kD; EC 1.6.99.3; CI-23kD; TYKY subunit |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Jurkat cell, mouse heart and brain tissue |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by antigen-affinity chromatography. |
| Buffer | 1XPBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 40% Glycerol |
| Concentration | 0.9 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O00217 Human NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial Swiss-port # Q8K3J1 Mouse NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial |
|---|---|
| Gene Symbol | NDUFS8 |
| Gene Full Name | NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) |
| Background | This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase, or Complex I, a multimeric enzyme of the respiratory chain responsible for NADH oxidation, ubiquinone reduction, and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and, as such, is required in the electron transfer process. Mutations in this gene have been associated with Leigh syndrome. |
| Function | Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). May donate electrons to ubiquinone. [UniProt] |
| Highlight | Related products: NDUFS8 antibodies; Anti-Rabbit IgG secondary antibodies; Related poster download: The Structure & Functions of Mitochondria.pdf |
| Research Area | Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 24 kDa |
Images (3) Click the Picture to Zoom In
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ARG65578 anti-NDUFS8 antibody WB image
Western blot: 40 μg of 1) Jurkat, 2) Mouse heart, and 3) Mouse brain lysates stained with ARG65578 anti-NDUFS8 antibody at 1:250 dilution. Exposure time: 20 seconds.
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ARG65578 anti-NDUFS8 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thyroid cancer tissue stained with ARG65578 anti-NDUFS8 antibody (left) at 1:20 dilution, or the same antibody preincubated with fusion protein (right). (Original magnification: ×200).
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ARG65578 anti-NDUFS8 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thyroid cancer tissue stained with ARG65578 anti-NDUFS8 antibody (left) at 1:20 dilution, or the same antibody preincubated with fusion protein (right). (Original magnification: ×200).
