ARG65289

anti-NDUFS8 antibody

anti-NDUFS8 antibody for Western blot and Mouse,Rat

Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes NDUFS8
Tested Reactivity Ms, Rat
Predict Reactivity Hu, Dog
Tested Application WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name NDUFS8
Antigen Species Human
Immunogen C-EAEIAANIQAD
Conjugation Un-conjugated
Alternate Names EC 1.6.5.3; TYKY; NADH-ubiquinone oxidoreductase 23 kDa subunit; CI-23k; NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial; CI23KD; Complex I-23kD; EC 1.6.99.3; CI-23kD; TYKY subunit

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.1 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 225887 Mouse NDUFS8

Swiss-port # Q8K3J1 Mouse NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

Background This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase, or Complex I, a multimeric enzyme of the respiratory chain responsible for NADH oxidation, ubiquinone reduction, and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and, as such, is required in the electron transfer process. Mutations in this gene have been associated with Leigh syndrome. [provided by RefSeq, Mar 2010]
Research Area Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 24 kDa

Images (1) Click the Picture to Zoom In

  • ARG65289 anti-NDUFS8 antibody WB image

    Western Blot: Mouse Heart lysate (35 µg protein in RIPA buffer) stained with ARG65289 anti-NDUFS8 antibody at 0.1 µg/ml dilution.