ARG65311

anti-NDUFS6 antibody

anti-NDUFS6 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes NDUFS6
Tested Reactivity Hu, Ms, Rat
Predict Reactivity Cow, Dog
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name NDUFS6
Antigen Species Human
Immunogen C-RIRFVGRQKEVNEN
Conjugation Un-conjugated
Alternate Names NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial; CI13KDA; CI-13kA; CI-13kD-A; Complex I-13kD-A; NADH-ubiquinone oxidoreductase 13 kDa-A subunit

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 µg/ml
WB0.01 - 0.03 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 29478 Rat NDUFS6

GeneID: 407785 Mouse NDUFS6

GeneID: 4726 Human NDUFS6

Background This gene encodes a subunit of the NADH:ubiquinone oxidoreductase (complex I), which is the first enzyme complex in the electron transport chain of mitochondria. This complex functions in the transfer of electrons from NADH to the respiratory chain. The subunit encoded by this gene is one of seven subunits in the iron-sulfur protein fraction. Mutations in this gene cause mitochondrial complex I deficiency, a disease that causes a wide variety of clinical disorders, including neonatal disease and adult-onset neurodegenerative disorders.[provided by RefSeq, Oct 2009]
Highlight Related products:
NDUFS6 antibodies; Anti-Goat IgG secondary antibodies;
Related poster download:
The Structure & Functions of Mitochondria.pdf
Research Area Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 14 kDa

Images (3) Click the Picture to Zoom In

  • ARG65311 anti-NDUFS6 antibody WB image

    Western Blot: Human Heart lysate (35 µg protein in RIPA buffer) stained with ARG65311 anti-NDUFS6 antibody at 0.01 µg/ml dilution.

  • ARG65311 anti-NDUFS6 antibody WB image

    Western Blot: Mouse (A) and Rat (B) Heart lysates (35 µg protein in RIPA buffer) stained with ARG65311 anti-NDUFS6 antibody at 0.1 µg/ml dilution.

  • ARG65311 anti-NDUFS6 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65311 anti-NDUFS6 antibody at 5 µg/ml dilution followed by AP-staining.