ARG65311
anti-NDUFS6 antibody
anti-NDUFS6 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Overview
Product Description | Goat Polyclonal antibody recognizes NDUFS6 |
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Tested Reactivity | Hu, Ms, Rat |
Predict Reactivity | Cow, Dog |
Tested Application | IHC-P, WB |
Host | Goat |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | NDUFS6 |
Antigen Species | Human |
Immunogen | C-RIRFVGRQKEVNEN |
Conjugation | Un-conjugated |
Alternate Names | NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial; CI13KDA; CI-13kA; CI-13kD-A; Complex I-13kD-A; NADH-ubiquinone oxidoreductase 13 kDa-A subunit |
Application Instructions
Application Suggestion |
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Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
Preservative | 0.02% Sodium azide |
Stabilizer | 0.5% BSA |
Concentration | 0.5 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Background | This gene encodes a subunit of the NADH:ubiquinone oxidoreductase (complex I), which is the first enzyme complex in the electron transport chain of mitochondria. This complex functions in the transfer of electrons from NADH to the respiratory chain. The subunit encoded by this gene is one of seven subunits in the iron-sulfur protein fraction. Mutations in this gene cause mitochondrial complex I deficiency, a disease that causes a wide variety of clinical disorders, including neonatal disease and adult-onset neurodegenerative disorders.[provided by RefSeq, Oct 2009] |
Highlight | Related products: NDUFS6 antibodies; Anti-Goat IgG secondary antibodies; Related poster download: The Structure & Functions of Mitochondria.pdf |
Research Area | Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody |
Calculated MW | 14 kDa |
Images (3) Click the Picture to Zoom In
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ARG65311 anti-NDUFS6 antibody WB image
Western Blot: Human Heart lysate (35 µg protein in RIPA buffer) stained with ARG65311 anti-NDUFS6 antibody at 0.01 µg/ml dilution.
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ARG65311 anti-NDUFS6 antibody WB image
Western Blot: Mouse (A) and Rat (B) Heart lysates (35 µg protein in RIPA buffer) stained with ARG65311 anti-NDUFS6 antibody at 0.1 µg/ml dilution.
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ARG65311 anti-NDUFS6 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65311 anti-NDUFS6 antibody at 5 µg/ml dilution followed by AP-staining.