ARG65577
anti-NDUFS5 antibody
anti-NDUFS5 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NDUFS5 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NDUFS5 |
| Antigen Species | Human |
| Immunogen | Fusion protein of human NDUFS5 |
| Conjugation | Un-conjugated |
| Alternate Names | CI-15k; CI-15 kDa; Complex I-15 kDa; NADH dehydrogenase [ubiquinone] iron-sulfur protein 5; CI15K; NADH-ubiquinone oxidoreductase 15 kDa subunit |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Human liver tissue and K562 cell, human fetal brain tissue and Raji cell |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by antigen-affinity chromatography. |
| Buffer | 1XPBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 40% Glycerol |
| Concentration | 2.8 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O43920 Human NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 |
|---|---|
| Gene Symbol | NDUFS5 |
| Gene Full Name | NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Q reductase) |
| Background | This gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. Alternative splicing results in multiple transcript variants and pseudogenes have been identified on chromosomes 1, 4 and 17. |
| Function | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [UniProt] |
| Highlight | Related products: Anti-Rabbit IgG secondary antibodies; Related poster download: The Structure & Functions of Mitochondria.pdf |
| Research Area | Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 13 kDa |
Images (3) Click the Picture to Zoom In
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ARG65577 anti-NDUFS5 antibody WB image
Western blot: 40 μg of 1) Human liver tissue, 2) K562, 3) Human fetal brain and Raji lysates stained with ARG65577 anti-NDUFS5 antibody at 1:400 dilution. Exposure time: 5 seconds.
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ARG65577 anti-NDUFS5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thyroid cancer tissue stained with ARG65577 anti-NDUFS5 antibody (left) at 1:50 dilution, or the same antibody preincubated with fusion protein (right). (Original magnification: ×200).
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ARG65577 anti-NDUFS5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thyroid cancer tissue stained with ARG65577 anti-NDUFS5 antibody (left) at 1:50 dilution, or the same antibody preincubated with fusion protein (right). (Original magnification: ×200).
