ARG65574
anti-NDUFS2 antibody
anti-NDUFS2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NDUFS2 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NDUFS2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide of human NDUFS2 |
| Conjugation | Un-conjugated |
| Alternate Names | Complex I-49kD; CI-49kD; CI-49; NADH-ubiquinone oxidoreductase 49 kDa subunit; EC 1.6.99.3; NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial; EC 1.6.5.3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Mouse heart and brain tissue, Jurkat cell |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by antigen-affinity chromatography. |
| Buffer | 1XPBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 40% Glycerol |
| Concentration | 3.1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O75306 Human NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial Swiss-port # Q91WD5 Mouse NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial |
|---|---|
| Gene Symbol | NDUFS2 |
| Gene Full Name | NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase) |
| Background | The protein encoded by this gene is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mutations in this gene are associated with mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Function | Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). [UniProt] |
| Highlight | Related products: NDUFS2 antibodies; Anti-Rabbit IgG secondary antibodies; Related poster download: The Structure & Functions of Mitochondria.pdf |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 53 kDa |
| PTM | Dimethylation at Arg-118 by NDUFAF7 takes place after NDUFS2 assembles into the complex I, leading to stabilize the early intermediate complex (PubMed:24089531, PubMed:24838397). |
Images (3) Click the Picture to Zoom In
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ARG65574 anti-NDUFS2 antibody WB image
Western blot: 40 μg of 1) Mouse heart, 2) Mouse brain and 3) Jurkat lysates stained with ARG65574 anti-NDUFS2 antibody at 1:400 dilution. Exposure time: 20 seconds.
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ARG65574 anti-NDUFS2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human ovarian cancer tissue stained with ARG65574 anti-NDUFS2 antibody (left) at 1:50 dilution, or the same antibody preincubated with synthetic peptide (right). (Original magnification: ×200).
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ARG65574 anti-NDUFS2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human ovarian cancer tissue stained with ARG65574 anti-NDUFS2 antibody (left) at 1:50 dilution, or the same antibody preincubated with synthetic peptide (right). (Original magnification: ×200).
