ARG65266
anti-NDUFS2 antibody
anti-NDUFS2 antibody for Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes NDUFS2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Cow, Dog, Pig |
| Tested Application | WB |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_004541.1; NP_001159631.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NDUFS2 |
| Antigen Species | Human |
| Immunogen | C-SRGVRQWQPDVE |
| Conjugation | Un-conjugated |
| Alternate Names | Complex I-49kD; CI-49kD; CI-49; NADH-ubiquinone oxidoreductase 49 kDa subunit; EC 1.6.99.3; NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial; EC 1.6.5.3 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The protein encoded by this gene is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mutations in this gene are associated with mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 53 kDa |
| PTM | Dimethylation at Arg-118 by NDUFAF7 takes place after NDUFS2 assembles into the complex I, leading to stabilize the early intermediate complex (PubMed:24089531, PubMed:24838397). |
Images (1) Click the Picture to Zoom In
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ARG65266 anti-NDUFS2 antibody WB image
Western Blot: Human Heart lysate (35 µg protein in RIPA buffer) stained with ARG65266 anti-NDUFS2 antibody at 0.1 µg/ml dilution.
