ARG65261
anti-NDUFS1 antibody
anti-NDUFS1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Controls and Markers antibody; Metabolism antibody; Neuroscience antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes NDUFS1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Cow, Rat, Dog |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NDUFS1 |
| Antigen Species | Human |
| Immunogen | C-TEKSATYVNTEGR |
| Conjugation | Un-conjugated |
| Alternate Names | CI-75k; EC 1.6.5.3; NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; CI-75Kd; EC 1.6.99.3; PRO1304; Complex I-75kD; CI-75kD |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P28331 Human NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial Swiss-port # Q91VD9 Mouse NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial |
|---|---|
| Background | The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011] |
| Research Area | Cancer antibody; Controls and Markers antibody; Metabolism antibody; Neuroscience antibody |
| Calculated MW | 79 kDa |
Images (2) Click the Picture to Zoom In
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ARG65261 anti-NDUFS1 antibody WB image
Western Blot: Human Heart lysate (35 µg protein in RIPA buffer) stained with ARG65261 anti-NDUFS1 antibody at 0.3 µg/ml dilution.
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ARG65261 anti-NDUFS1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human heart tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65261 anti-NDUFS1 antibody at 5 µg/ml dilution followed by AP-staining.
