ARG40047

anti-NDUFB7 antibody

anti-NDUFB7 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes NDUFB7
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NDUFB7
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-137 of Human NDUFB7 (NP_004137.2).
Conjugation Un-conjugated
Alternate Names NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7; CI-B18; NADH-ubiquinone oxidoreductase B18 subunit; Complex I-B18; Cell adhesion protein SQM1; B18

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:100
IHC-P1:50 - 1:100
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse heart and HL-60
Observed Size 16 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4713 Human NDUFB7

GeneID: 66916 Mouse NDUFB7

Swiss-port # P17568 Human NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

Swiss-port # Q9CR61 Mouse NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

Gene Symbol NDUFB7
Gene Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa
Background The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It is located at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [provided by RefSeq, Jul 2008]
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [UniProt]
Cellular Localization Mitochondrion inner membrane; Peripheral membrane protein. Mitochondrion intermembrane space. [UniProt]
Calculated MW 16 kDa

Images (1) Click the Picture to Zoom In

  • ARG40047 anti-NDUFB7 antibody WB image

    Western blot: 25 µg of Mouse heart and HL-60 cell lysates stained with ARG40047 anti-NDUFB7 antibody at 1:3000 dilution.