ARG40061
anti-NDUFB4 antibody
anti-NDUFB4 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NDUFB4 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NDUFB4 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-90 of Human NDUFB4 (NP_001161803.1). |
| Conjugation | Un-conjugated |
| Alternate Names | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4; CI-B15; B15; NADH-ubiquinone oxidoreductase B15 subunit; Complex I-B15 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Mouse liver and HeLa | ||||||||
| Observed Size | 15 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O95168 Human NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 Swiss-port # Q9CQC7 Mouse NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 |
|---|---|
| Gene Symbol | NDUFB4 |
| Gene Full Name | NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4, 15kDa |
| Background | This gene encodes a non-catalytic subunit of the multisubunit NADH:ubiquinone oxidoreductase, the first enzyme complex in the mitochondrial electron transport chain (complex I). Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to ubiquinone. [provided by RefSeq, Dec 2009] |
| Function | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [UniProt] |
| Cellular Localization | Mitochondrion inner membrane; Single-pass membrane protein; Matrix side. [UniProt] |
| Calculated MW | 15 kDa |
Images (2) Click the Picture to Zoom In
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ARG40061 anti-NDUFB4 antibody WB image
Western blot: 25 µg of Mouse liver and HeLa cell lysates stained with ARG40061 anti-NDUFB4 antibody at 1:3000 dilution.
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ARG40061 anti-NDUFB4 antibody WB image
Western blot: Extracts from normal (control) and NDUFB4 knockout (KO) HeLa cells, using ARG40061 anti-NDUFB4 antibody at 1:1000 dilution.
