ARG40142
anti-NDUFB3 antibody
anti-NDUFB3 antibody for Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NDUFB3 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NDUFB3 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide between aa. 13-45 of Human NDUFB3. |
| Conjugation | Un-conjugated |
| Alternate Names | Complex I-B12; NADH-ubiquinone oxidoreductase B12 subunit; B12; CI-B12; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | HeLa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide. |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O43676 Human NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 Swiss-port # Q9CQZ6 Mouse NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 |
|---|---|
| Gene Symbol | NDUFB3 |
| Gene Full Name | NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 3, 12kDa |
| Background | This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which is the first enzyme in the electron transport chain of mitochondria. This protein localizes to the inner membrane of the mitochondrion as a single-pass membrane protein. Mutations in this gene contribute to mitochondrial complex 1 deficiency. Alternative splicing results in multiple transcript variants encoding the same protein. Humans have multiple pseudogenes of this gene. [provided by RefSeq, Mar 2012] |
| Function | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [UniProt] |
| Cellular Localization | Mitochondrion inner membrane; Single-pass membrane protein; Matrix side. [UniProt] |
| Calculated MW | 11 kDa |
Images (1) Click the Picture to Zoom In
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ARG40142 anti-NDUFB3 antibody WB image
Western blot: 20 µg of HeLa whole cell lysate stained with ARG40142 anti-NDUFB3 antibody at 1:2000 dilution.
